Using massively parallel sequencing the genome sequence can be accurately determined. Depending on your setting and interest the whole genome can be sequenced (even starting from a very limited amount of DNA) or specific genes or regions (e.g. exome sequencing) can be targeted.
Transcriptomics (or expression profiling) is the study of the complete set of RNA transcripts that are produced by specific cells or tissues under specific circumstances. Messenger RNAs (mRNA), micro RNAs (miRNA), long non-coding RNAs (lncRNA), etc can be sequenced and quantified, allowing differential gene expression analysis.
Epigenetics research seeks to describe dynamic alterations in the transcriptional potential of a cell. The main epigenetic mechanisms are DNA methylation, histone modification, DNA accessibility and non-coding RNA-associated gene silencing. NXTGNT has all the tools in house to analyse these epigenetic mechanism in an integrated analysis.
NXTGNT can help you with your sequencing data analysis with a team of high-skilled experts. We perform data analysis starting from raw sequencing reads, working towards publication-ready results: Statistics, visualization, PCA analysis, Gene-Set Enrichment Analysis, pathway analysis, etc. Feel free to contact us regarding any question.
10X Genomics single cell and linked-reads
We provide all single cell and linked-reads sequencing solutions offered by the 10X Genomics Chromium System & GemCode Technology
Cancer precision medicine
Molecular insights, connecting physicians and their patients to the latest cancer treatment approaches through pharmacogenetics, making precision medicine a reality.
Oxford nanopore sequences unamplified DNA and RNA, eliminating the need for amplification steps (and the resulting quantitative and qualitative amplification bias). Long reads up to 100 kbp with direct detection of 5-methylcytosines in DNA and N6-methyladenines in RNA provide tremendous advantages in de novo assembly, detection of large structural variants, RNA isomforms, etc.
NXTGNT was founded in 2008 to create a rich and unique substrate for groundbreaking research, development and valorization at Ghent University. The main goal of NXTGNT is the establishment of a reference center for next generation sequencing (NGS), (epi-)genome and transcriptome analysis at Ghent University. NXTGNT established a unique service platform by the integration of a state-of-the-art NGS library preparation facility with several sequencing platforms and a strong expertise in -omics data-analysis (bioinformatics).