Genomics is the study of the full genetic complement of an organism (the genome). By using massively parallel sequencing this genome sequence can be accurately and precisely determined. Depending on your setting and interest the whole genome can be sequenced (even starting from a very limited amount of DNA) or specific genes or regions can be targeted.
Epigenetics research seeks to describe dynamic alterations in the transcriptional potential of a cell. Changes in the epigenome occur regularly and naturally, but can also be influenced by several factors including age, the environment/lifestyle, and disease state. Epigenomics is believed to be the link between the environment and gene expression. The main epigenetic mechanisms are DNA methylation, histone modification and non-coding RNA-associated gene silencing. NXTGNT has all the tools in house to analyse these epigenetic mechanism in an integrated analysis.
Transcriptomics (or expression profiling) is the study of the transcriptome – the complete set of RNA transcripts that are produced by specific cells or tissues under specific circumstances. Depending on your interest several different RNA transcripts, messenger RNAs (mRNA), micro RNAs (miRNA), long non-coding RNAs (lncRNA), etc can be profiled. By using high-throughput methods, such as massively parallel sequencing, one can compare samples’ transcriptomes and identify transcripts (genes) that are differentially expressed between groups of samples.
With the ever increasing throughput in sequencing technology, data analysis remains a challenge for which high-skilled experts are imperative. NXTGNT can help you if you do not have the necessary in-house expertise or when you want to out-source and speed-up the analysis of your data. We perform, in collaboration with ProGenTomics, data analysis starting from raw sequencing reads (filtering, trimming, mapping of reads), data normalization and statistics, visualization, PCA analysis, Gene-set enrichment analysis, Pathway analysis, etc. Feel free to contact us regarding any question.
NXTGNT was founded in 2008 to create a rich and unique substrate for groundbreaking research, development and valorization at Ghent University. The main goal of NXTGNT is the establishment of a reference center for next generation sequencing (NGS), (epi-)genome and transcriptome analysis at Ghent University. NXTGNT establised a unique service platform by the integration of a state-of-the-art NGS library preparation facility with several sequencing platforms and a strong expertise in -omics data-analysis (bioinformatics). NXTGNT's final goal is the establishment of an innovative genome analysis service facility with clear focus on