Whole Genome Amplification

Typically, whole genome sequencing requires an input amount of at least 0.1 µg of DNA. If for some reason, your available DNA is limited, the DNA can be amplified before being sequenced by using Whole Genome Amplification (WGA). Currently, we routinely apply the SurePlex DNA Amplification system which yields 2 to 5 µg of DNA from input DNA as low as 10 to 50 pg. These amounts can be obtained from only a few cells.

 

Advantages of Whole Genome Amplification

  • Low input amounts of DNA (10 - 50 pg) can be amplified to sufficient amounts for sequencing

 

Whole Genome Amplification workflow 

For this small amount of sample, a quality control is not possible. We start directly with the DNA amplification followed by preparing the sequencing libraries by adding indexes and primer adapters. After a quality assessment step these libraries are sequenced on one of our Illumina sequencers. The technical quality of the sequencing run is monitored in real time. 

Sequencing data can be transferred to you via the Illumina BaseSpace platform or our own server (FTP download).

 

Feel free to contact us with any questions.

Contact Us

NXTGNT, UGent sequencing reference center

 Ghent University, Faculty of Pharmaceutical Sciences

  Ottergemsesteenweg 460, 9000 Gent, Belgium

 

  +32 9 264 80 59