Equipment

State-of-the-art sample preparation

NXTGNT possesses state-of-the-art sample preparation equipment that allows to do a lot of different sample preparations in an automated, efficient and accurate way.

 

Diagenode IP-Star

 
The Diagenode IP-Star is a compact automated system that provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental conditions. It allows for high-throughput sample preparation compatible with popular downstream applications such as qPCR and next generation sequencing platforms. In addition to automated ChIP and DNA methylation protocols, this system contains a wide variety of protocols including next generation sequencing library preparation and sample purifications.
 

Covaris S2

The Covaris S2 focused-ultrasonicator is a versatile, high power system engineered for pre-analytical sample processing for numerous biological and chemical applications including, DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization. The computer controlled device allows for shearing at a certain, set length.

 

State-of-the-art High-throughput library preparation and quality control

NXTGNT is fully equipped with the latest technologies to process a high throughput of samples with the most diverse library preparation protocols. A ZEISS PALM MicroBeam Axio Observer 7 allows laser capture microdissection. Virtually all steps are controlled for quality using Qubit Fluorometric Quantitation (Thermo Fisher Scientific), q-PCR, Bioanalyser (Agilent Genomics), LabChip (PerkinElmer), etc.

 

 

 

Our Sequencers

NXTGNT offers a wide range of sequencing apparatus allowing to sequence at the scale and scope of your project

 

MiSeq

 
The in-house MiSeq benchtop sequencer is well suited for smaller projects and thus allows for more focused applications such as targeted gene sequencing, metagenomics, small genome and transcriptome sequencing, and targeted gene expression. Generating 1 million up to 25 million reads, the MiSeq sequencer is ideal for reduced representation studies and narrowly focused applications.
More MiSeq specifications
 
 
 
 
 
 
 
NextSeq 500

With an output of up to 400 million reads, our in-house Illumina NextSeq 500 sequencer is more suited for larger study designs. The NextSeq 500 is easily configured providing researchers perfect scalability for mid-sized to large projects at maximal operational efficiency.
More NextSeq500 specifications.

 

 

HiSeq 3000

The HiSeq 3000 System is a powerful and efficient high-throughput sequencing system that, with an output of up to 750 gigabase (Gb) of data, is ideally suited for large-scale genomics projects.
More HiSeq 3000 specifications.

 

 

 

 

Novaseq 6000

The Novaseq 6000 System provides scalable ultra-high-throughput and flexibility for virtually any sequencing method, genome, and scale of project.
More Novaseq 6000 specifications.

 

 

PacBio Sequel

The PacBio RS is a Single Molecule, Real-Time (SMRT) DNA Sequencing System that provides the highest consensus accuracy and longest read lengths of, on average, up to 10,000 bp which makes it extermely suitable for whole-genome sequencing, de novo assemblies and metagenomics of viral populations. Moreover, SMRT sequencing can directly detect epigenetic modifications, making it useful in epigenetics studies.

 

 

 

 

10X Genomics Chromium Controller 

The Chromium Controller has been designed to rapidly and efficiently automate highly parallel sample partitioning and molecular barcoding. The Chromium Controller allows a user to run any Chromium Solution, spanning from genome to single cell analysis.