December 15, 2017

Directional RNA-seq (total RNA, mRNA, miRNA)

NXTGNT offers directional RNA sequencing of total RNA, mRNA, and miRNA (small RNA). RNA sequencing not only provides comprehensive knowledge about all transcripts present in the sample, but also quantifies the abundance of each transcript. With directional RNA sequencing, information is retained concerning the strand (sense vs anti-sense) the RNA originated from. The choice for mRNA, miRNA or total RNA sequencing depends on your setting and question you want to answer.

RNA-seq advantages

  • Full transcriptome from every species
  • Differential gene/transcript expression analysis
  • Directionality of the RNA is retained (sense vs antisense strand)

RNA-seq workflow

You provide us with extracted total RNA samples. After an initial quality check of the RNA which determines concentration and integrity of the RNA, sequencing libraries from the samples are obtained. We only continue with samples that passed this initial quality check. If for some reason certain samples did not pass this initial check, we will contact you to discuss on how to proceed.

Depending on the kind of RNA-seq a size-selection step is performed. Also the library preparation may vary depending on the kind of RNA-seq. The prepared libraries undergo a final quality check before being loaded for sequencing on one of our Illumina sequencers. The technical quality of the sequencing run is monitored in real time.

Sequencing data can be transferred to you via the Illumina BaseSpace platform or our own server (FTP download).

For more information, please contact us.