With targeted (re)sequencing, a subset of genes or regions of the genome is isolated and sequenced. Targeted approaches using next-generation sequencing allow researchers to focus time, expenses, and data analysis on specific areas of interest. Such targeted analysis can include for instance include specific genes of interest, targets within genes, or mitochondrial DNA.
Advantages of Targeted (Re)Sequencing
- Focuses on regions of interest, generating a smaller, more manageable data set
- Reduces sequencing costs and data analysis burdens
- Reduces turnaround time compared to broader approaches
- Enables deep sequencing at high coverage levels for rare variant identification
Exome and Targeted (Re)Sequencing workflow
First, we will perform a quality analysis of the DNA samples provided to us by you. We will need at least 1µg of DNA. Only when the provided genomic DNA is of sufficient quality and quantity will we proceed with the next steps. In case the DNA provided to us is of insufficient quality and/or quantity, we will contact you and discuss how to proceed.
Then, your samples will be enriched for the set of genes or regions of your interest by bead capturing. From these enriched samples sequencing libraries are prepared by adding indexes and primer adapters. After another quality assessment step these libraries are sequenced on one of our Illumina Sequencers. The technical quality of the sequencing run is monitored in real time.
Sequencing data can be transferred to you via the Illumina BaseSpace platform or our own server (FTP download).
Feel free to contact us for more information.